PRODUCT

SHORELINE BIOME

(주)엠디엑스케이는 고객의 연구 목적에 적합한 기술과 서비스를 제공합니다.

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Brand

The most comprehensive results from
the simplest microbiome sequencing prep.

Shoreline biome의 all-in-one, easy-to-use microbiome assay kits로 lysis부터 바코드로
multiplexed된 결과분석까지 지원함으로써 빠르고 쉽게 high-quality microbiome data를 얻을 수 있습니다.

Technology

  • Patented (patent pending)
    Lysis/DNA Purification

    • Novel lysis cracks microbes other kits miss

  • Novel and proprietary
    primer design

    • Considered structure and function of ribosomal
      RNA to ensure complete microbe coverage
    • Reduces amplification bias, leading to more
      accurate bacterial representation

  • Unique dual end barcodes

    • Reducing index hopping and increasing
      usable reads

Shoreline Complete™ assay kits

  • Assay kits for illumina sequencing platform

    Targeting either the V1–V3 or V4 region of the 16S rRNA gene

  • Assay kits for PacBio sequencing platform

    Targeting either the full 16S rRNA gene or the 16S-ITS-23S region

SB analyzer software

Features

  • Strain level microbial identification using Shoreline Biome’s custom
    Athena database and StrainID amplicon

    Categorizes novel strains

    Uniquely-curated database of well-annotated genomes

  • SBanalyzer software demultiplexes and maximizes usable reads

    Intuitive analytical tool with point-and-click interface

    Athena database from RefSeq genomes (currently contains
    18,566 genomes and 77,212 16S-23S regions)

Workflow

The Process

Advantages / Features

Advantages

  • Cell Lysis

    일반적인 DNA preparation 방법으로
    용해하기 어려운 미생물에도 적용가능

  • PCR Sequencing Targets

    16S rRNA 미생물 유전자를 타겟으로 하여
    multiplex primer 설계 및 검증, 많은 sample (>100)을
    단일 런으로 동시에 sequencing할 수 있도록 최적화

  • Data Analysis

    SBanalyzer software with Athena database
    바코드로 샘플을 분류하고 Athena™ reference
    database를 활용한 자체 software로 strain 수준으로
    pylogenetic identification 가능

Features

  • Easy and quick workflow

    Superior lysis with no bead beating

    Low sample input for precious samples

  • Smarter PCR primer design reduces bias

    Unique dual-ended barcodes maximizes usable reads

    High fidelity single-step PCR

  • Analysis software powered by
    reference database

    Improve accuracy & resolution for PacBio NGS

    Athena™ reference database groups into taxonomic hierarchy
    for strain level ID

  • Various applicable sample types

    Stool, skin, swab, tissue

    Soil, insect, dairy

Applications

Microbiome-wide associations Translational Research

Resources

KMB MDxK workshop

6월 23일에서 25일까지 개최되는2021년 한국미생물 • 생명공학회 국제학술대회 및 정기학술대회에서엠디엑스케이 워크샵을 진행합니다.Shoreline Biome사의..

2021-06-22

Shoreline Breaker: Fast HMW DNA Purification from Fecal, Bacteria and ..

Shoreline Breaker: Fast HMW DNA Purification from Fecal, Bacteria and Spore SamplesLong read sequenc..

2021-04-21

Comparing Two Sequencing Tools for Evaluating Live Biotherapeutics as ..

Microbiome연구에서의 극복해야할 과제는 장내 미생물이 인간의 건강과 질병에 미치는 영향 및 메커니즘을 이해하고 효능을 평가하는 데 필요한 분류학적 해상도를 달성하는 것입..

2021-02-25
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