PRODUCT

Navinci Diagnostics

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Next-generation immunostaining of cells and tissues

in situ protein analysis.

Navinci Diagnostics는 단백질 간의 interaction 및 단백질의 modification, expression을 확인할 수 있는 Proximity Ligation Assay를 제공합니다.

Technology

Proximity ligation with Navenibodies

Probe incubation
:Unfolding Proximity Probe가
결합된 2차 항체 처리
Probe activation
Template formation
:Ligase를 넣어
Circle 형태로 만듦
Signal amplification
:rolling circle amplification
Visualization and data processing
:형광현미경 관찰

Easy sample-to-results workflow

Obtain distinct signals with NaveniFlex's streamlined workflow
Review and capture images using a fluorescent microscope
Perform digital quantification with imaging software

Workflow

Advantage / Features

Advantage

  • Clear target detection

    단백질의 과발현없이 현미경으로 직접 발현확인
    Higher Signal to Noise ratio : Reduced tissue background

  • Sensitive in situ detection

    Endogenous 상태에서 발현량이 약하거나 일시적인 단백질 결합 확인 가능
    Save precious antibodies

  • Reliable supply and quality

    Consistency in staining
    Reproducibility

  • Straightforward protocol and workflow

    No picture enhancement needed
    기존의 immunofluorescense 이미징 장비를 이용한 시각화

Features

  • Investigate unmodified cells and tissue

    Detect proteins in their native state, without any overexpression or modifications.

  • Spatial (contextual) analysis

    Retain protein localization : Visualize proteins as they are

  • High specificity

    Two antibodies detect two different epitopes on the same protein or complex

  • Excellent signal resolution

    Obtain clear target detection

  • Digitally quantify data

    Works with several image analysis software’s- dot counting

  • Assay within one day

    6 hour protocol NaveniFlex assay

  • High-quality reagents

    Navenibodies : carefully selected antibodies conjugated to proprietary oligo arms

  • High sensitivity

    NaveniFlex unique probe design significantly enhances the signal

Applications

Protein expresstion

* Possibility to detect low abundant proteins

Protein modification

* Specific detection of post-translational modifications, also with the use of pan-specific antibodies

Protein interaction

* Detection of protein-protein interactions

Protein detection
PDL1
Cells
HDLM-2, SignalSlide® PD-L1 IHC Controls 13747
Detection fluorophore
TEX615
NaveniFlex MM
Detection of phosphorylation
of PDGFR-ß
Cells
fresh frozen, PFA fixed BJhTert cells
Detection fluorophore
TEX615
NaveniFlex MR
Protein interaction
of proximity between H3K4me3 & H3K27me3
Cells
Neuronal Stem/Precursor Cells (primof proximity between H3K4me3 & H3K27me3 ary cultures)
Detection fluorophore
Atto647N
NaveniFlex MR
Protein detection
beta catenin and E-Cadherin
Tissue
FFPE Skin Tissue
Detection fluorophore
TEX615
NaveniFlex GR
Protein interaction
of beta-catenin and e-cadherin
Tissue
Human skin tissue
Detection fluorophore
ATTO488
NaveniFlex MR
Protein interaction
between CD8a & HLAa
Tissue
Lung cancer FFPE tissue
Detection fluorophore
ATTO488
NaveniFlex MR

Research Area

  • Immuno-profiling
  • Treatment validation
  • Signaling profiling
  • Biomarker discovery
  • Drug testing
  • Diagnostics

Resources

단백질 간의 Interaction, Modification, Expression을 확인할 수 있는 새로운 platform ..

NaveniFlex Proximity Ligation Assay Reagent KitNaveniFlex는 단백질 간의 Interaction 및 단백질의 Modification, E..

2021-01-20
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