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NGS validation with RNA in situ Hybridization

2018-08-08 09:44:06

PUBLICATION NEWSLETTER 

 

 

 RNAscope® assay validates all major findings! 

 

 

 

 

 

Validate single cell transcriptomic information in-situ: Mouse intestinal epithelium 

Haber, A. L., et al. (2017). Nature . DOI: 10.1038/nature24489 

 

RNAseq transcriptome profiling of 53,193 individual epithelial cells enabled the identification and characterization of previously unknown subtypes of intestinal epithelial cell and their gene signatures.

 

   

 

Single cell preparation from mouse intestine 

  


Transcriptome analysis 

 


Validating and mapping in situ 

 

 

 

- Validating and localizing newly identified cell type marker in single cells within tissue environment


 

 
 
- Validating regional cell-type diversity



 
 
- Validating and mapping newly identified subset of cells in situ 
 

 

 

 


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