The RNAscope VS Assay is designed for use with the Discovery Ultra and Discovery XT Automated IHC/ISH slide staining systems by Ventana Medical Systems. The RNAscope® VS Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides.
The RNAscope VS Assay workflow requires the following from ACD: - RNAscope VS Target Probes - RNAscope VS Control Probes - RNAscope VS Control Slides (optional) - RNAscopeVS 2.0 Chromogenic Reagent Kit-BROWN or RNAscope®VS 2.0 Chromogenic Reagent Kit-RED
Each RNAscope 2.0 HD Reagent Kit (Brown or Red) provides enough reagents to stain ~60 tissue sections approximately 20 mm x 20 mm large. Larger tissue sections will consume larger amount of reagents, resulting in fewer tests per kit. Each RNAscopeVS Reagent Kit contains three sub-kits: a Pretreatment Kit, a Detection Kit, and Wash Buffer and can be purchased individually. The stained slides are visualized with brightfield microscopes.
The RNAscope® VS Assay workflow requires the following from Ventana Medical Systems: - 760-224 mRNA DAB Detection Kit - 760-223 mRNA Pretreatment Kit - 760-222 mRNA Probe Amplification Kit - 760-225 (one part number that will deliver all three kits 224, 223, 222) - 960-761 thru 960-785 User Fillable Probe Dispenser
KEY FEATURES AND BENEFITS
1. Walk-away automation with reduced hands-on time 2. High reproducibility and consistency from run to run 3. High throughput and productivity
- Drug development and research applications: Target selection and validation, cellular disease models, Preclinical safety assessment, pharmacodynamics studies, RNA biomarker development and companion diagnostics research, and retrospective patient stratification - Cancer research applications: Tumor heterogeneity, Tumor Microenvironment (TME), Circulating Tumor Cells, Fusion Genes- Stem cell research: Identification of stem cells and signaling molecules, stem cell functions in vivo, stem cell signaling, stem cells and disease association - Neuroscience, Cell Biology, Infectious diseases, Immunology