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  • 제품명RNAscope® 2.5 HD Duplex Reagent Kit

    제조사ACD

    Cat No.322430

    규격ea

    첨부자료

RNAscope® 2.5 HD Duplex Reagent Kit, is a RNA in situ hybridization (ISH) chromogenic assay designed for multiplexing up to two targets. It uses HRP (Green) and Fast Red chromogenic stains and the probe names often include C1 or C2. It is ideal for simultaneous detection of two RNA species using RNA ISH. Each RNAscope® Duplex Reagent Kit provides enough reagents to stain ~20 tissue sections, each with an area of approximately 20 mm x 20 mm (0.75in x 0.75in).

Each kit contains:

RNAscope Duplex Detection Kit (PN 322500)

RNAscope Pretreatment Reagents (PN 322000, 322330)

RNAscope Wash Buffer (PN 310091).


KEY FEATURES AND BENEFITS
1. Simultaneous detection of two RNA species
2. Single-molecule detection
3. Ready to use reagents
4. Ability to visualize spatial patterns of two RNAs with single cell resolution

APPLICATIONS
- Drug development and research applications: Target selection and validation, cellular disease models, Preclinical safety assessment, pharmacodynamics studies, RNA biomarker development and companion diagnostics research, and retrospective patient stratification
- Cancer research applications: Tumor heterogeneity, Tumor Microenvironment (TME), Circulating Tumor Cells, Fusion Genes
- Stem cell research: Identification of stem cells and signaling molecules, stem cell functions in vivo, stem cell signaling, stem cells and disease association
- Neuroscience, Cell Biology, Infectious diseases, Immunology

 

Sample Type Compatibility

Adherent cells, Cell pellets, FFPE, Fixed frozen tissue, Freshfrozen tissue, TMA

 

RNAScope Workflow

  • Permeabilize
  • Hybridize
  • Amplify
  • Visualize