The RNAscope Leica Assay is designed as an automated assay on the Leica BOND RX platform. The RNAscope® Leica Assay, is an RNA in situ hybridization assay based on ACD’s patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in a multitude of sample types mounted on slides.
The RNAscope Leica Assay workflow requires the following from ACD: - RNAscope LS Target Probes - RNAscope Leica Assay Reagent Kit-BROWN - RNAscope Leica Assay Control Probes Pack (recommended not required) - RNAscope FFPE Control Slide Pack Hs, RNAscope FFPE Control Slide Pack Ms(recommended not required)
Each RNAscope Leica Assay Reagent Kit (Brown) provides enough reagents to stain ~60 tissue sections approximately 20 mm x 20 mm large. Larger tissue sections will consume larger amount of reagents, resulting in fewer tests per kit. Each RNAscope Leica Assay Reagent Kit contains three sub-kits: a Pretreatment Kit, a Detection Kit, and Wash Buffer. The fully automated assay includes tissue preparation, pretreatment and amplification steps and final hematoxylin staining all on the instrument, requiring minimal hands-on time. The stained slides are visualized with brightfield microscopes.
The RNAscope Leica Assay workflow requires the following from Leica Biosystems: - Bond Open Containers 30 mL - Bond Universal Convertiles 100 pack - Bond Epitope Retrieval Solution 1-1L (RTU) - Bond Epitope Retrieval Solution 2-1L (RTU) - Bond Dewax Solution 1L (RTU) - Bond Wash Solution 10X Concentrate - Bond Polymer Refine Detection (DAB) and Hematoxylin - 30 mL each - Bond Aspirating Probe Cleaning System KEY FEATURES AND BENEFITS
1. Walk-away automation with reduced hands-on time 2. High reproducibility and consistency from run to run 3. High throughput and productivity
- Drug development and research applications: Target selection and validation, cellular disease models, Preclinical safety assessment, pharmacodynamics studies, RNA biomarker development and companion diagnostics research, and retrospective patient stratification - Cancer research applications: Tumor heterogeneity, Tumor Microenvironment (TME), Circulating Tumor Cells, Fusion Genes- Stem cell research: Identification of stem cells and signaling molecules, stem cell functions in vivo, stem cell signaling, stem cells and disease association - Neuroscience, Cell Biology, Infectious diseases, Immunology