The RNAscope Multiplex Fluorescent Assay is based on ACD patented signal amplification and background suppression technology. The assay uses a novel and proprietary method of in situ hybridization (ISH) to visualize single RNA molecules per cell in fresh frozen tissue samples mounted on slides. RNAscope Assays do not require the RNA-free environment used for traditional ISH. This RNA ISH assay provides the same exceptional sensitivity as our singleplex assays, allowing single-molecule detection of up to three RNA targets simultaneously and is ideal for co-localization studies of any expressed gene set in nearly any tissue type. Up to 4 fluorescent labels are used including FITC (green), Cy3 (orange), Cy5 (red) and Cy7 (far red).
The RNAscope ISH assay is compatible with a variety of sample types including fresh frozen or formalin fixed parrafin embedded slides or tissue blocks, fixed frozen slides, chamber slides contains adherent cells, peripheral blood mononuclear cells and circulating tumor cells.
The RNAscope Multiplex Fluorescent Assay workflow requires - RNAscope Target Probe - RNAscope Control Probes - RNAscope Control Slides (optional) - RNAscope Multiplex Fluorescent Reagent Kit
Each RNAscope Multiplex Fluorescent Reagent Kit provides enough reagents to stain ~20 tissue sections approximately 20 mm x 20 mm large. Larger tissue sections will result in fewer tests. Each kit contains three sub-kits (a Pretreatment Kit, a Detection Kit, and Wash Buffer). Key Features and Benefits 1. Simultaneous in situ detection of up to four RNA species 2. Single-molecule detection 3. Ready-to-use reagents 4. Compatible with fresh tissue, FFPE, Cells