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RNAscope Automated Assay

RNAscope Automated Assay

ACD

RNA in situ reagent kit for use with automated tissue staining systems from the Bond RX System from Leica Biosystems or DISCOVERY ULTRA and DISCOVERY XT Automated Platforms from Roche Tissue Diagnostic

제품특징

Features

  • Best-in-class RNA ISH for your automated systems of choice
  • High throughput and automated RNA in situ hybridization (ISH) analysis


Applications

  • a fully automated walk-away solution on the Leica BOND RX System by Leica Biosystems automating the Permeabilize, Hybridize and Amplify steps of the RNAscope® Workflow
  • automated walk-away solution on the DISCOVERY ULTRA and DISCOVERY XT automated tissue staining systems by Roche Tissue Diagnostics automating the Hybridize and Amplify steps of the RNAscope® Workflow


For Leica systems




Roche DISCOVERY ULTRA system








RNAscope/BaseScope/ miRNAscope Assay Selection Guide

 

 RNAscope Assay

BaseScope Assay

miRNAscope Assay 

 

 

 

 

Number of ZZ Pair per target

 

  • Standard probe design is 20 ZZ probes (minimum of 6 ZZ probes)

 

 

  • 1 to 3 ZZ probes based on the application

 

 

  • N/A

 

 Target

  • mRNA >300 bases
  • lncRNA > 300 bases

 

  • Exon junctions/Splice Variants
  • RNA 50 to 300 bases
  • Validated point mutations

 

 

  • Small RNAs 17-50 bases ASOs, miRNAs, siRNAs

 

Application

 

  • lcnRNA and mRNA >300 bases

 

 

  •  Exon junctions/splice variants, circular RNA, gene fusion, gene knockout
  • Short/highly homologous sequences, TCRs and CDR sequence for T cell clones, pre-miRNA, gene editing/CRISPR, CAR-T cell validation and detection
  • Point mutation, short InDel, homologuesa

 

 

  • Small RNAs 17-50 bases 
  • ASOs, miRNAs, piRNAs, shRNAs, siRNAs, and tRNAs

 

Multiplex capability 

 

  •  Single to up to 12-plex

 

 

  • Single to Duplex 

 

 

  • Single-plex 

 

 Detection

 

  •  Chromogenic or fluorescent

 

 

  •  Chromogenic

 

 

  •  Chromogenic

 

 Sample type

  • FFPE cells & tissues (TMAs)
  • Fresh frozen tissues
  • Fixed frozen tissues
  • Cultured cells
  • FFPE cells & tissues (TMAs)
  • Fresh frozen tissues
  • Fixed frozen tissues
  • Cultured cells
  • FFPE cells & tissues (TMAs)
  • Fresh frozen tissues
  • Fixed frozen tissues
  • Cultured cells

 Pretreatment kit

 

  • Reagent Kit includes Universal Pretreatment Kit 

 

 

  • Reagent Kit includes Universal Pretreatment Kit 

 

 

  •  Reagent Kit includes Universal Pretreatment Kit 

 

 Kit

  • RNAscope kit  
  • BASEscope kit 
  • miRNAscope kit 

 Probe

  • RNAscope Probe 
  • BASEscope probe 
  •  miRNAscope probe 

 Automation

  • Available on Leica Bond Rx and Ventana Discovery ULTRA 
  • Singleplex available on Leica Bond Rx and Ventana Discovery ULTRA 
  • Available on Leica Bond Rx 

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품목정보

품번 품명 가격
322100 RNAscope® 2.5 LS Reagent Kit – BROWN 견적문의
322150 RNAscope® 2.5 LS Reagent Kit – RED 견적문의
322440 RNAscope® 2.5 LS Duplex Assay (BROWN/RED) 견적문의
322800 RNAscope® LS Multiplex Fluorescent Assay 견적문의
322200 RNAscope® 2.5 VS Reagent Kit – BROWN 견적문의
322250 RNAscope® 2.5 VS Reagent Kit – RED 견적문의
323200 RNAscope® VS Universal HRP 견적문의
323250 RNAscope® VS Universal AP 견적문의
323300 RNAscope® VS Duplex Assay 견적문의